the effect of beta interferon on dendritic cells and cytokine synthesis by cd4+ t cells

نویسندگان

saeid abediankenari

department of microbiology and immunology, faculty of medicine, mazandaran university of medical sciences, sari, iran davoud shaker

department of microbiology and immunology, faculty of medicine, mazandaran university of medical sciences, sari, iran farshideh abedian

department of microbiology and immunology, faculty of medicine, mazandaran university of medical sciences, sari, iran arazmohammad mirabi

department of microbiology and immunology, faculty of medicine, mazandaran university of medical sciences, sari, iran

چکیده

background: dendritic cells (dc) are a key regulator of the immune response, and interferon- beta (ifn-β) is considered an immunomodulatory molecule for dc. objective: the purpose of this study was to evaluate the ability of ifn-β treated dc to induce cytokine secretion by cd4+ t cells. methods: dendritic cells were generated from blood monocytes with granulocyte-monocyte colony-stimulating factor and interleukin-4 with or without ifn-β. we analyzed the production of cd4+ t helper cytokines (il-17, ifn- γ and il-10) in the supernatant of the dendritic cell-t cell co- cultures by elisa. we also studied the effects of hla-g and costimulatory molecules on immature and mature dc. results: ifn-γ and il-17 decreased significantly in the presence of hla-gbearing dc compared to control cultures (p

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عنوان ژورنال:
iranian journal of immunology

جلد ۶، شماره ۲، صفحات ۶۱-۶۶

کلمات کلیدی
[ ' d e n d r i t i c c e l l s ' , ' i n t e r f e r o n ' , ' b e t a ' , ' i n t e r l e u k i n ' , 1 7 ]

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